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南京农业大学资源与环境科学学院导师:张瑞福正文
姓名:张瑞福
电话:025--84396212
职称:教授
邮箱:
从事专业:植物营养与土壤微生物学
研究方向:根际微生物学与生物技术
院系:植物营养系
简介:
张瑞福,山东泰安人,1974年出生于山东省泰安市。2004年4月在南京农业大学获理学博士学位, 2004年6月起先后在香港大学、美国德克萨斯农工大学、加州大学戴维斯分校从事博士后研究。2010年4月起任南京农业大学资源与环境科学学院教授,博士生导师。SCI 杂志《International Biodeterioration & Biodegradation》(IBB) 的编委。中国微生物学会环境微生物专委会委员。南京农业大学高层次引进人才。主要从事根际微生物与生物技术研究,研究领域包括根际微生物学、微生物有机肥、土壤微生物多样性及其优化调控、土壤有机污染物的微生物降解、木质纤维素的微生物酶解等
教育及工作经历:
1994.9-1998.6 山东莱阳农学院 农学系 农学学士
1998.9-2004.4 南京农业大学微生物学系 硕博连读 理学博士
2004.6-2006.6 Department of Biodiversity & Ecology, Hong Kong University, Postdoctoral Research Fellow
2006.6-2008.9 Department of Chemical Engineering and Department of Microbiology & Plant Pathology, Texas A&M University, Postdoctoral Research Associate.
2008.9-2010.5 Department of Biological & Agricultural Engineering, University of California, Davis,Postdoctoral Research Associate
2010.5- 南京农业大学资源与环境科学学院 教授 博士生导师。
研究方向:
(1)根际微生物工程:主要从事根际有益微生物在根际的生态行为、效应及定殖的分子机理、与植物根系之间的分子互作与信号调控;集约化农田与高强度连作保护蔬菜地以及新开补充耕地的快速培肥过程中,高产及抑病型根际土壤微生物区系的构建与调控措施、效果;功能性微生物有机肥的开发及其在可持续、低投入农业上的应用。
(2)微生物高效木质纤维素酶制剂的开发及协同作用机理:主要从事高效木质纤维素降解菌的分离,木质纤维素酶的分离纯化、协同作用机理,基因克隆、高效表达(E.coli, B.subtilis, Phichia pastoris, Nuerospa crass, Aspergillus niger等作为表达宿主),酶的定向进化、高效基因工程菌的构建、及工业用高效酶制剂的开发等。
主持课题:
(1) “根际促生菌Bacillus amyloliquefaciens SQR9与植物根系分泌物互作的分子机理研究”,41271271,国家自然科学基金面上项目,2013.1-2016.12.75万元,主持。
(2) “生防菌Bacillus amyloliquefaciens SQR9在植株根表原位条件下形成生物膜的蛋白组变化研究”,20110097110002,教育部博士点博导基金,2012.1-2014.12,12万元,主持。
(3) “微生物有机肥产业发展中的关键技术研究”,2011BAD11B03,科技部科技支撑计划,2011.1-2015.12, 255万元,课题主持。
(4) “利用有机(类)肥料调控我国土壤微生物区系关键技术研究”,201103004,农业部公益性行业科研专项,2011.1-2015.12,83万元,骨干参加。
(5) “高温木质纤维素酶制剂的研发及协同作用机理研究”,03-06J0425,中央高校基本科研业务费,2010.11-2012.11,40万元,主持。
(6) “南京农业大学高层次引进人才科研启动经费”,680-804103,南京农业大学,2010.5-2015.5,50万元,主持。
(7) “青年骨干人才培养与引进计划”03-80900205,江苏省优势学科建设项目,2011.7-2012.7,10万元,主持。
(8) “有机磷农药水解酶基因水平转移的分子基础研究”,30400014,国家自然科学青年基金, 2005.1-2007.12,20万元,主持。
(9) “农业资源与环境学科生物学研究创新引智基地(111项目)”,B12009,教育部、国家外国专家局,2012.1-2016.12, 中方骨干参加。
(10) “新型农业微生物肥效制剂的创制,2013AA100802,科技部863项目,2013.1-2017.12,900万元,主持。
专利与奖励:
(1) 美国专利,Zhiliang Fan, Takao Kasuga, Weihua Wu, Xiaochao Xiong, Ruifu Zhang,2012,Biochemical platform for fuels and chemicals production from cellulosic biomass,US Patent: 20120129221A1
(2) 国家发明专利,李顺鹏;张瑞福;蒋建东;戴青华;崔中利;何健.2005.一种三唑磷农药残留降解菌及其生产的菌剂.CN200410044951.5.
(3) 国家发明专利,李顺鹏;顾立锋;何健;洪青;张瑞福;蒋建东.2006 一种辛硫磷农药残留降解菌及其生产的菌剂CN200510022545.3.
(4) 国家科技进步二等奖,李顺鹏、崔中利、沈标、刘智、何健、杨新民、王新华、张瑞福、蒋建东、洪青.2005.农药残留微生物降解技术的研究与应用。
发表文章:
2012
28.Huang X, Chaparro JM, Reardon KF, Zhang R, Shen Q and Vivanco JM.2012.Root exudates and microbial diversity in the rhizopshere.Environmental Microbiology.(Editor invited review, In revision)
27.Zhang J, Shen Z, Yuan J, Wang B, Shen Q and Zhang R*.2012.Inclusive analysis of the biocontrol mechanisms of Trichoderma harzianum SQR-T037, an efficient biocontrol agent for banana wilt.Applied Microbiology & Biotechnology (In revision)
26.Xu Z, Shao J, Li B, Yan X, Shen Q and Zhang R*.2012.Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to antifungal activity and biofilm formation Applied and Environmental Microbiology (Pressed online, DOI:10.1128/AEM.02645-12) (Nominated by Editors as the Spotlight article)
25.Weng J†, Wang Y†, Li J, Shen Q and Zhang R*.2012. Enhancing root colonization and biocontrol activity of Bacillus amyloliquefaciens SQR9 by abrB gene disruption Applied Microbiology & Biotechnology (Pressed online, DOI: 10.1007/s00253-012-4572-4)
24.Ding C, Shen Q, Zhang R, and Chen W*.2012.Evaluation of rhizosphere bacteria and derived bio-organic fertilizers as potential biocontrol agents against bacterial wilt (Ralstonia solanacearum) of potato.Plant Soil.(Pressed online, DOI: 10.1007/s11104-012-1425-y)
23.Li S, Zhang N, Zhang Z, Luo J, Shen B, Zhang R and Shen Q*.2012.Antagonist Bacillus subtilis HJ5 controls Verticillium wilt of cotton by root colonization and biofilm formation.Biol Fertil Soils (Pressed online, DOI: 10.1007/s00374-012-0718-x) (IF3yr=2.319)
22.Qiu M†, Zhang R†, Xue C, Zhang S, Li S, Zhang N and Shen Q*.2012.Application of a novel bio-organic fertilizer can control Fusarium wilt by regulating the microbial community of cucumber rhizosphere soils.Biol Fertil Soils 48:807-816.(Co-First author)
21.Liao H, Xu C, Tan S, Wei Z, Ling N, Yu G, Raza W, Zhang R, Shen Q and Xu Y*.2012.Production and characterization of acidophilic xylanolytic enzymes from Penicillium oxalicum GZ-2.Bioresour Technol.123:117-124 .
20.Zhang R*, Pan L, Zhao Z and Gu J-D*.2012 High Incidence of Plasmids in Marine Vibrio Species Isolated from Mai Po Nature Reserve of Hong Kong Ecotoxicology, 21:1661-1668 (Co-corresponding author)
19.Wang Y, Weng J, Raza W, Yin X, Zhang R and Shen Q*.2012.Bacillus subtilis genome editing using ssDNA with short homologous fragments.Nucleic Acids Res.1-9.
18.Huo Z, Zhang N, Waseem Raza, Shen Q and Zhang R*.2012.Comparison of the spores of Paenibacillus polymyxa SQR-21 prepared at different temperatures.Biotechnology Letters 34:925-933.
17.Fan Z*, Wu W, Hildebrand A,Kusuga T,Zhang R and Xiong X.2012.A novel biochemical platform for fuels and chemicals production from cellulosic biomass.Plos One 7:e31693.
16.Huo Z†, Zhang N†, Xu Z, Li S, Zhang Q, Qiu M, Huang Q, Zhang R* and Shen Q.2012.Optimization of survival and spore formation of Paenibacillus polymyxa SQR-21 during bioorganic fertilizer storage.Bioresour Technol.108:190-195.
15.Liu D, Zhang R, Yang X, Zhang Z, Song S, Miao Y and Shen Q*.2012.Characterization of a thermostable beta-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33.Microbial Cell Factories.11:25.
2011
14.Zhang N, Wu K, He X, Li S, Zhang Z, Shen B, Yang X, Zhang R, Huang Q and Shen Q*.2011.A new bioorganic fertilizer can effectively control banana wilt by strong colonization with Bacillus subtilis N11.Plant Soil.344:87-97.
13.Liu D, Zhang R, Yang X, Wu H, Xu D, Tang Z and Shen Q*.2011.Thermostable cellulase production of Aspergillus fumigatus Z5 under solid-state fermentation and its application in degradation of agricultural wastes.International Biodeterioration & Biodegradation 65(5):717-725.
12.Liu D, Zhang R, Yang X, Xu Y, Tang Z, Tian W and Shen Q*.2011.Expression, purification and characterization of two thermostable endoglucanases cloned from a lignocellulosic decomposing fungi Aspergillus fumigatus Z5 isolated from compost.Protein Expr Purif.79(2):176-86.
11.Liu D, Zhang R, Wu H, Xu D, Tang Z, Yu G, Xu Z and Shen Q*.2011.Changes in biochemical and microbiological parameters during the period of rapid composting of dairy manure with rice chaff.Bioresour Technol.102(19):9040-9.
10.Zhang R, Fan Z* and Kusuga T.2011.Heterologous expression of cellobiose dehydrogenase from Neurospora crassa in Pichia pastoris and its purification and characterization. Protein Expr Purif.75:63-69.
2009-2010
9.Zhang R, Lipuma JJ and Gonzalez CF*.2009 Two type IV secretion systems with different functions in Burkholderia cenocepacia K56-2.Microbiology 155: 4005-13.
8.Zhang R and Gu J-D*.2009.Complete sequence of plasmid pMP-1 from the marine environmental Vibrio vulnificus and location of its replication origin.Marine Biotechnology. 11(4):456-62.
2007-2008
7.Zhang R, Wang Y, Leung P and Gu J-D*.2007.pVC, a small, cryptic plasmid from the environmental isolate of Vibrio cholerae MP-1.J Microbiol.45:193-198.
6.Jiang, J., Zhang R, Cui Z, He J, Gu L and Li S*.2007.Parameters controlling the gene targeting frequency in Sphingomonas species rrn site and expression of the methyl parathion hydrolase.Journal of Applied Microbiology.102:1578-1585.
5.Jiang, J., Zhang R, Li R, Gu J-D and Li S*.2007 Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon.Biodegradation, 18:403-412.
2006
4.Zhang, R., Wang Y and Gu J-D*.2006.Identification of environmental plasmid-bearing Vibrio species isolated from polluted and pristine marine environments of Hong Kong and resistance to antibiotics and mercury. Anton Leeuw Int J G, 89:307-315.
3.Zhang, R., Jiang J, Gu J-D and Li S*.2006.Long term effect of methylparathion contamination on soil microbial community diversity estimated by 16S rRNA gene cloning. Ecotoxicology, 15:523-530.
2.Zhang, R., Cui Z, Zhang X, Jiang J, Gu J-D and Li S*.2006.Cloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer.Biodegradation, 17:465-472.
2005
1.Zhang, R., Cui Z, Jiang J, He J, Gu X and Li S*.2005.Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.Can J Microbiol.51(4): 337~343.
中文文章
16.张瑞福, 沈其荣* 2012.抑病型土壤的微生物区系特征及调控.南京农业大学学报.35:125-132.
15.戴青华, 张瑞福,蒋建东, 邱珊莲,李顺鹏* 2007.三唑磷水解酶基因的克隆及水解产物的确定.中国环境科学6:777-780
14.张瑞福,吴旭平,樊奔,何健,蒋建东,李顺鹏* 2005.有机磷农药污染土壤中有机磷农药降解菌的分离与多样性研究,生态学报.25(6):1502-1508.
13.张瑞福,蒋建东, 代先祝,顾立锋,李顺鹏* 2005.污染环境中降解基因的水平转移及其在生物修复中的作用, 遗传.27(5):845-851.
12.蒋建东,张瑞福,何健,张小舟, 崔中利, 李顺鹏* 2005.细菌对环境污染物的趋化性及其在生物修复中的作用, 生态学报.25(7):1764-1771.
11.戴青华, 张瑞福,蒋建东,顾立锋,李顺鹏* 2005.一株三唑磷降解菌mp-4的分离鉴定及降解特性的研究, 土壤学报.42(1):111-115.
10.张瑞福,戴青华,何健,代先祝,李顺鹏* 2004.七株有机磷农药降解菌的降解特性比较研究, 中国环境科学.24(5):584-587.
9.张瑞福,崔中利,李顺鹏* 2004.土壤微生物群落结构研究方法进展,土壤.36(5):476-480.
8.张瑞福,崔中利,何健,黄婷婷,李顺鹏* 2004.甲基对硫磷长期污染对土壤微生物的生态学效应, 农村生态环境.20(4):1-5.
7.张瑞福,蒋建东,崔中利,李顺鹏* 2004.菌株DLL-1降解土壤和韭菜中甲基对硫磷的研究,应用生态学报.15(2):295-298
6.蒋建东,曹慧,张瑞福,张明星,李顺鹏* 2004.有机磷农药对韭菜虫害的防治效果及农药的微生物降解,应用生态学报.15(8):1459-1462.
5.顾立锋,何健,张瑞福,李顺鹏* 2004.一株耐盐苯酚降解菌的分离及降解特性研究,土壤学报.41(5):756-760.
4.张瑞福,曹慧,崔中利,李顺鹏*,樊奔 2003.土壤微生物总DNA的提取与纯化,微生物学报.43(2):276-282.(截止到2012.9.1,已被引用78次,是《微生物学报》自1953年创刊以来被引次数最多的单篇论文,获第六届中国科协期刊优秀论文奖)
3.张瑞福,朱卫,崔中利,李顺鹏* 2003.辛硫磷降解菌X-1的分离鉴定及降解性状的初步研究,环境科学学报.23(3):411-413.
2.朱卫,张瑞福,崔中利,李顺鹏* 2003.一株杀虫单降解细菌的筛选与生物学特性研究,南京农业大学学报.26(1):100-103.
1.崔中利,张瑞福,何健,李顺鹏* 2002.对硝基苯酚降解菌P3的分离、降解性质及基因工程菌的构建,微生物学报.42(1):19-26.
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