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湖南农业大学硕士研究生导师介绍:王国梁正文
王国梁博士,男,1961年9月15日生。教授,作物遗传育种博士生导师。1982年7月于湖南农业大学本科毕业,获植物遗传学学士学位;1985年7月于福建农业大学硕士毕业,获植物遗传学硕士学位;1992年6月于获国际水稻研究所(IRRI)博士学位(Ph.D);1992年6月至1993年8月:美国Texas A&M University博士后;
1993年8月至1996年6月:美国University of California at Davis 博士后;
1996年6月至1999年9月:新加坡国立大学分子农业生物学院水稻抗病研究室主任(Principal Investigator and Senior Scientist);
1999年9月至 2004年4月:美国The Ohio State University助教授(Assistant Professor)
2004年4月至今:美国The Ohio State University副教授(Associate Professor)
2004年3月至今:湖南农业大学芙蓉学者特聘教授。
主要研究领域
水稻抗病基因的分离、克隆;植物-病原微生物相互作用的分子机制;植物基因组学
Our main research interest is to understand the genetic and molecular mechanism of plant resistance to pathogens. Both genetic and genomic approaches are being used in our laboratory. We are currently using rice as a model plant to clone disease resistance genes and genes involved in resistance responses to rice blast and bacterial blight pathogens. In particular, we are interested in understanding the molecular basis of broad-spectrum resistance to rice blast resistance genes. Two recently cloned blast resistance genes [Pi2(t) and Pi9(t)] in our lab are excellent materials for this research purpose since both genes confer a high level of resistance to diverse isolates. Understanding the relationship between programmed cell death and disease resistance is another focus in our lab. We have recently cloned the Spl11 gene that controls onset of lesion mimic formation in rice. Detailed analysis of the gene function will provide new insights into the mechanism of cell death in plants.
Using different genomics approaches, we are interested in understanding the transcriptome of resistance reaction to rice blast. Over 67,000 ESTs and 600,000 SAGE tags have been sequenced and analyzed. Many novel defense genes have been identified. Functional analysis of these genes in disease resistance is in progress using several new techniques such as microarrays, reverse genetics and transgenic analysis. Recently, we are using the new technology MPSS to comprehensively characterize the rice genome. Our ultimate goal is to genetically engineer crop plants for disease resistance in such a way as to reduce the use of environmentally damaging pesticides.
Email: Wang.620@osu.edu
主要代表作
1) Gowda M, Jantasuriyarat C,Dean R.,Wang GL (2004)Robust-LongSAGE (RL-SAGE):A Substantially Improved LongSAGE Method for Gene Discovery and Transcriptome Analysis. Plant Physiology,134: 890-897. (通讯作者)
2) Liu G,Lu G, Zeng L,Wang GL (2002)。Two broad-spectrum blast resistance genes,Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Molecular Genetics and Genomics,267:472-480. (通讯作者)
3) Wang GL,Ruan DL,Song WY, Sideris S,Chen L,Pi L,Whalen MC,Zhang S,Fauquet C,Ronald PC (1998) Xa21D encodes a receptor-like molecule with a Leucine-Rich-Repeat domain that determines race specific recognition and is subject to adaptive evolution. The Plant Cell 10:765-780.
4) Song WY, Wang GL,(co-first author共同第一作者)Chen L, Kim HS, Holsten T,Wang B, Zhai W, Zhu LH,Fauquet C,Ronald PC (1995) A receptor kinase-like protein encoded by the rice disease resistance gene, Xa-21. Science 270:1804-1806.
5) Wang GL,Holsten TE, Song WY, Wang HP, Ronald PC (1995) Construction of a rice bacterial artificial chromosome library and identification of clones linked to the Xa-21 disease resistance locus. The Plant Journal 7:525-533.
学术成果
在水稻抗病分子标记,抗病基因克隆方面成就突出,国际上克隆出水稻第一个基因Xa21基因,该基因是一类新的抗病基因,具有重要价值,相关论文发表在著名科学杂志Science上,该文至今被引用436次,该基因已被多个国家和地区应用于水稻育种与生产;克隆出水稻第一个MAPK基因——BWMK1, 克隆出细胞程序化调亡基因Spl11和RANK1基因,首次发现U-盒和ARM重复序列与细胞死亡及防卫信号反应有关; 国际上首次克隆出稻瘟病广谱抗性基因Pi2与Pi9等基因,为阐明植物抗病信号传导、广谱抗性分子机理奠定了重要基础;是BAC、TAC文库建立的主要贡献者。国际上率先开展水稻抗病基因组研究,获国际专利6项。以第一作者或通讯作者在Science, the Plant Cell, Plant Physiology, Plant Journal等SCI源刊上发表SCI收录论文近20篇,主要论文被SCI引用1200多次。
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